Western blot analysis of NFkB p52 CRISPR knockout HeLa (NFkB p52 KO) and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti NFkB p52 antibody (VMA00579) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 300 second and (B) 30 second exposure.